{"id":16585,"date":"2019-06-17T14:00:16","date_gmt":"2019-06-17T05:00:16","guid":{"rendered":"https:\/\/www.euglena.jp\/news\/?p=16585"},"modified":"2021-04-26T11:43:54","modified_gmt":"2021-04-26T02:43:54","slug":"20190617-2","status":"publish","type":"post","link":"https:\/\/www.euglena.jp\/news\/20190617-2\/","title":{"rendered":"\u30df\u30c9\u30ea\u30e0\u30b7\u3067\u306e\u9ad8\u52b9\u7387\u30b2\u30ce\u30e0\u7de8\u96c6\u306b\u6210\u529f"},"content":{"rendered":"

\u30df\u30c9\u30ea\u30e0\u30b7\u3067\u306e\u9ad8\u52b9\u7387\u30b2\u30ce\u30e0\u7de8\u96c6\u306b\u6210\u529f
\n\uff5e\u30d0\u30a4\u30aa\u71c3\u6599\u751f\u7523\u306a\u3069\u306b\u5411\u3051\u305f\u6709\u7528\u30df\u30c9\u30ea\u30e0\u30b7\u80b2\u7a2e\u3078\u671f\u5f85\uff5e<\/p>\n

\u682a\u5f0f\u4f1a\u793e\u30e6\u30fc\u30b0\u30ec\u30ca
\n\u7406\u5316\u5b66\u7814\u7a76\u6240<\/p>\n

\u3000<\/strong>\u7406\u5316\u5b66\u7814\u7a76\u6240\uff08\u7406\u7814\uff09\u74b0\u5883\u8cc7\u6e90\u79d1\u5b66\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u30d0\u30a4\u30aa\u751f\u7523\u60c5\u5831\u7814\u7a76\u30c1\u30fc\u30e0 \u306e\u91ce\u6751\u4fca\u5c1a\u7814\u7a76\u54e1\u3001\u6301\u7530\u6075\u4e00\u30c1\u30fc\u30e0\u30ea\u30fc\u30c0\u30fc\u3001\u79d1\u6280\u30cf\u30d6\u7523\u9023\u672c\u90e8\u30d0\u30c8\u30f3\u30be\u30fc\u30f3\u7814\u7a76\u63a8\u9032\u30d7\u30ed\u30b0\u30e9\u30e0\u5fae\u7d30\u85fb\u985e\u751f\u7523\u5236\u5fa1\u6280\u8853\u7814\u7a76\u30c1\u30fc\u30e0\u306e\u9234\u6728\u5065\u543e\u30c1\u30fc\u30e0\u30ea\u30fc\u30c0\u30fc\uff08\u682a\u5f0f\u4f1a\u793e\u30e6\u30fc\u30b0\u30ec\u30ca\u3000\u57f7\u884c\u5f79\u54e1\u7814\u7a76\u958b\u767a\u62c5\u5f53\/\u5148\u7aef\u6280\u8853\u7814\u7a76\u90e8\u9577\uff09\u3089\u306e\u7814\u7a76\u30c1\u30fc\u30e0\u306f\u3001\u30df\u30c9\u30ea\u30e0\u30b7\u306e\u7523\u696d\u5229\u7528\u7a2eEuglena gracilis <\/em>[1]<\/sup>\uff08\u4ee5\u4e0b\u3001\u30df\u30c9\u30ea\u30e0\u30b7\uff09\u3092\u5bfe\u8c61\u3068\u3057\u305f\u9ad8\u52b9\u7387\u306e\u30b2\u30ce\u30e0\u7de8\u96c6[2]<\/sup>\u65b9\u6cd5\u306e\u78ba\u7acb\u306b\u521d\u3081\u3066\u6210\u529f\u3057\u307e\u3057\u305f\u3002
\n\u3000<\/strong>\u672c\u7814\u7a76\u6210\u679c\u306f\u3001\u30df\u30c9\u30ea\u30e0\u30b7\u306e\u57fa\u790e\u7814\u7a76\u306e\u63a8\u9032\u3084\u3001\u6709\u7528\u682a\u306e\u80b2\u7a2e\u306b\u5927\u304d\u304f\u8ca2\u732e\u3059\u308b\u3068\u671f\u5f85\u3067\u304d\u307e\u3059\u3002
\n\u3000<\/strong>\u30df\u30c9\u30ea\u30e0\u30b7\u306f\u3001\u98df\u54c1\u3084\u30d0\u30a4\u30aa\u71c3\u6599\u3078\u306e\u5fdc\u7528\u304c\u9032\u3081\u3089\u308c\u3066\u3044\u308b\u6709\u7528\u306a\u5fae\u7d30\u85fb\u985e\u3067\u3059\u3002\u3057\u304b\u3057\u3001\u305d\u306e\u57fa\u790e\u7814\u7a76\u3084\u6709\u7528\u682a\u80b2\u7a2e\u3092\u9032\u3081\u308b\u4e0a\u3067\u91cd\u8981\u3067\u3042\u308b\u52b9\u7387\u7684\u306a\u30b2\u30ce\u30e0\u7de8\u96c6\u6cd5\u306f\u3001\u3053\u308c\u307e\u3067\u78ba\u7acb\u3055\u308c\u3066\u3044\u307e\u305b\u3093\u3067\u3057\u305f\u3002
\n\u3000<\/strong>\u4eca\u56de\u3001\u7814\u7a76\u30c1\u30fc\u30e0\u306f\u3001\u30df\u30c9\u30ea\u30e0\u30b7\u7d30\u80de\u306bCas9 RNP\u8907\u5408\u4f53[3]<\/sup>\u3092\u76f4\u63a5\u5c0e\u5165\u3059\u308b\u624b\u6cd5\u3092\u7528\u3044\u3066\u3001\u6700\u9069\u306a\u6761\u4ef6\u3092\u898b\u3044\u3060\u3059\u3053\u3068\u306b\u3088\u308a\u3001\u7d0480 \uff05\u3068\u3044\u3046\u975e\u5e38\u306b\u9ad8\u3044\u7de8\u96c6\u52b9\u7387\u3067\u6a19\u7684\u907a\u4f1d\u5b50\u3078\u306e\u6b20\u640d\u30fb\u633f\u5165\u5909\u7570\u5c0e\u5165\u306b\u6210\u529f\u3057\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u30012\u7a2e\u985e\u306eCas9 RNP\u8907\u5408\u4f53\u3092\u7528\u3044\u305fDNA\u306e\u7279\u5b9a\u9818\u57df\u3078\u306e\u9577\u3044\u6b20\u640d\u5909\u7570\u306e\u5c0e\u5165\u3084\u3001\u4e00\u672c\u9396\u306e\u30c9\u30ca\u30fcDNA\u5206\u5b50\u3092\u7528\u3044\u305f\u6b63\u78ba\u306a\u5916\u6765DNA\u5206\u5b50\u306e\u5c0e\u5165\u3082\u53ef\u80fd\u3067\u3042\u308b\u3053\u3068\u3092\u660e\u3089\u304b\u306b\u3057\u307e\u3057\u305f\u3002
\n\u3000<\/strong>\u672c\u7814\u7a76\u306f\u3001\u82f1\u56fd\u306e\u79d1\u5b66\u96d1\u8a8c\u300ePlant Biotechnology Journal<\/em>\u300f\u306e\u63b2\u8f09\u306b\u5148\u7acb\u3061\u3001\u30aa\u30f3\u30e9\u30a4\u30f3\u7248\uff085\u670826\u65e5\u4ed8\u3051\uff09\u306b\u63b2\u8f09\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n

\"\u56f3\uff11\"<\/p>\n

\u56f3\u3000\u4eca\u56de\u6210\u529f\u3057\u305f\u30df\u30c9\u30ea\u30e0\u30b7\u306b\u304a\u3051\u308bCas9 RNP\u3092\u7528\u3044\u305f\u30b2\u30ce\u30e0\u7de8\u96c6\u624b\u6cd5\u306e\u6982\u8981<\/p>\n

\u203b\u7814\u7a76\u30c1\u30fc\u30e0<\/strong>
\n\u7406\u5316\u5b66\u7814\u7a76\u6240
\n\u3000<\/strong>\u74b0\u5883\u8cc7\u6e90\u79d1\u5b66\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u3000\u30d0\u30a4\u30aa\u751f\u7523\u60c5\u5831\u7814\u7a76\u30c1\u30fc\u30e0
\n\u3000\u3000<\/strong>\u7814\u7a76\u54e1\u3000\u91ce\u6751\u3000\u4fca\u5c1a\u3000\uff08\u306e\u3080\u3089\u3000\u3068\u3057\u3072\u3055\uff09
\n\u3000\u3000\uff08<\/strong>\u7406\u7814 \u79d1\u6280\u30cf\u30d6\u7523\u9023\u672c\u90e8 \u30d0\u30c8\u30f3\u30be\u30fc\u30f3\u7814\u7a76\u63a8\u9032\u30d7\u30ed\u30b0\u30e9\u30e0\u3000\u5fae\u7d30\u85fb\u985e\u751f\u7523\u5236\u5fa1\u6280\u8853\u7814\u7a76\u30c1\u30fc\u30e0\u3000\u7814\u7a76\u54e1\uff09
\n\u3000\u3000<\/strong>\u30c6\u30af\u30cb\u30ab\u30eb\u30b9\u30bf\u30c3\u30d5II\u3000\u4e95\u4e0a\u3000\u5c0f\u69d9\u3000\uff08\u3044\u306e\u3046\u3048\u3000\u3053\u307e\u304d\uff09
\n\u3000\u3000<\/strong>\u30c6\u30af\u30cb\u30ab\u30eb\u30b9\u30bf\u30c3\u30d5II\u3000\u4e0a\u539f (\u5c71\u53e3) \u3000\u7531\u7d00\u5b50\u3000\uff08\u3046\u3048\u306f\u3089 (\u3084\u307e\u3050\u3061)\u3000 \u3086\u304d\u3053\uff09
\n\u3000\u3000<\/strong>\u30c1\u30fc\u30e0\u30ea\u30fc\u30c0\u30fc\u3000\u6301\u7530\u3000\u6075\u4e00\u3000\uff08\u3082\u3061\u3060\u3000\u3051\u3044\u3044\u3061\uff09
\n\u3000\u3000\uff08<\/strong>\u7406\u7814 \u79d1\u6280\u30cf\u30d6\u7523\u9023\u672c\u90e8 \u30d0\u30c8\u30f3\u30be\u30fc\u30f3\u7814\u7a76\u63a8\u9032\u30d7\u30ed\u30b0\u30e9\u30e0\u3000\u5fae\u7d30\u85fb\u985e\u751f\u7523\u5236\u5fa1\u6280\u8853\u7814\u7a76\u30c1\u30fc\u30e0\u3000\u526f\u30c1\u30fc\u30e0\u30ea\u30fc\u30c0\u30fc\uff09
\n\u79d1\u6280\u30cf\u30d6\u7523\u9023\u672c\u90e8\u3000\u30d0\u30c8\u30f3\u30be\u30fc\u30f3\u7814\u7a76\u63a8\u9032\u30d7\u30ed\u30b0\u30e9\u30e0
\n\u3000<\/strong>\u5fae\u7d30\u85fb\u985e\u751f\u7523\u5236\u5fa1\u6280\u8853\u7814\u7a76\u30c1\u30fc\u30e0
\n\u3000\u3000<\/strong>\u5ba2\u54e1\u7814\u7a76\u54e1\u3000\u5c71\u7530\u3000\u5eb7\u55e3\u3000\uff08\u3084\u307e\u3060\u3000\u3053\u3046\u3058\uff09
\n\u3000\u3000<\/strong>\uff08\u682a\u5f0f\u4f1a\u793e\u30e6\u30fc\u30b0\u30ec\u30ca \u5148\u7aef\u6280\u8853\u7814\u7a76\u90e8 \u5148\u7aef\u6280\u8853\u7814\u7a76\u8ab2\u3000\u30c1\u30fc\u30e0\u30ea\u30fc\u30c0\u30fc\uff09
\n\u3000\u3000<\/strong>\u5ba2\u54e1\u4e3b\u7ba1\u7814\u7a76\u54e1\u3000\u5ca9\u7530\u3000\u4fee\u3000\uff08\u3044\u308f\u305f\u3000\u304a\u3055\u3080\uff09
\n\u3000\u3000<\/strong>\uff08\u682a\u5f0f\u4f1a\u793e\u30e6\u30fc\u30b0\u30ec\u30ca \u7d4c\u55b6\u6226\u7565\u90e8\uff09
\n\u3000\u3000<\/strong>\u30c1\u30fc\u30e0\u30ea\u30fc\u30c0\u30fc \u3000\u9234\u6728\u3000\u5065\u543e\u3000\uff08\u3059\u305a\u304d\u3000\u3051\u3093\u3054\uff09
\n\u3000\u3000<\/strong>\uff08\u682a\u5f0f\u4f1a\u793e\u30e6\u30fc\u30b0\u30ec\u30ca\u3000\u57f7\u884c\u5f79\u54e1\u7814\u7a76\u958b\u767a\u62c5\u5f53\/\u5148\u7aef\u6280\u8853\u7814\u7a76\u90e8\u9577\uff09<\/p>\n

\uff11\uff0e\u80cc\u666f<\/strong><\/p>\n

\u3000<\/strong>\u8fd1\u5e74\u3001\u6301\u7d9a\u53ef\u80fd\u6027\u3068\u7d4c\u6e08\u6d3b\u52d5\u3092\u4e21\u7acb\u3059\u308b\u30d0\u30a4\u30aa\u30a8\u30b3\u30ce\u30df\u30fc\u306e\u5b9f\u73fe\u3084\u3001\u6301\u7d9a\u53ef\u80fd\u306a\u958b\u767a\u76ee\u6a19\uff08SDGs\uff09[4]<\/sup>\u306e\u9054\u6210\u306b\u5411\u3051\u3066\u3001\u5fae\u7d30\u85fb\u985e\u3092\u7528\u3044\u305f\u30d0\u30a4\u30aa\u71c3\u6599\u3084\u30d0\u30a4\u30aa\u30d7\u30e9\u30b9\u30c1\u30c3\u30af\u306a\u3069\u306e\u6709\u7528\u7269\u8cea\u751f\u7523\u6280\u8853\u306e\u958b\u767a\u304c\u671f\u5f85\u3055\u308c\u3066\u3044\u307e\u3059\u3002
\n\u3000<\/strong>\u306a\u304b\u3067\u3082\u3001\u5c4b\u5185\u5916\u3067\u306e\u5927\u91cf\u57f9\u990a\u6cd5\u304c\u78ba\u7acb\u3055\u308c\u3066\u3044\u308b\u30df\u30c9\u30ea\u30e0\u30b7\u306e\u7523\u696d\u5229\u7528\u7a2eEuglena gracilis<\/em>\uff08\u4ee5\u4e0b\u3001\u30df\u30c9\u30ea\u30e0\u30b7\uff09\u306f\u3001\u597d\u6c17\u6761\u4ef6\u4e0b\u306b\u304a\u3044\u3066\u3001\u30d0\u30a4\u30aa\u30d7\u30e9\u30b9\u30c1\u30c3\u30af\u306e\u6750\u6599\u3084\u6a5f\u80fd\u6027\u98df\u54c1\u3068\u306a\u308b\u8caf\u8535\u591a\u7cd6\u30d1\u30e9\u30df\u30ed\u30f3[5]<\/sup>\u3092\u84c4\u7a4d\u3057\u307e\u3059\uff08\u56f31\uff09\u3002\u4e00\u65b9\u3001\u5acc\u6c17\u6761\u4ef6\u4e0b\u3067\u306f\u3001\u30d1\u30e9\u30df\u30ed\u30f3\u304b\u3089\u30ef\u30c3\u30af\u30b9\u30a8\u30b9\u30c6\u30eb\u3068\u547c\u3070\u308c\u308b\u6cb9\u8102\u3092\u7523\u751f\u3057\u307e\u3059\u3002\u30ef\u30c3\u30af\u30b9\u30a8\u30b9\u30c6\u30eb\u306f\u30b8\u30a7\u30c3\u30c8\u6a5f\u71c3\u6599\u306b\u9069\u3057\u3066\u304a\u308a\u3001\u73fe\u5728\u3001\u5b9f\u7528\u5316\u306b\u5411\u3051\u305f\u8a66\u9a13\u304c\u9032\u3081\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u307e\u305f\u3001\u30df\u30c9\u30ea\u30e0\u30b7\u306f\u6804\u990a\u4fa1\u304c\u9ad8\u304f\u3001\u4e16\u754c\u7684\u306b\u9700\u8981\u304c\u62e1\u5927\u3057\u3064\u3064\u3042\u308b\u98fc\u990c\u6599\u3068\u3057\u3066\u3082\u6ce8\u76ee\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u80cc\u666f\u304b\u3089\u3001\u30df\u30c9\u30ea\u30e0\u30b7\u3092\u5229\u6d3b\u7528\u3059\u308b\u305f\u3081\u306e\u751f\u7269\u5b66\u7684\u77e5\u8b58\u306e\u96c6\u7a4d\u3068\u3001\u6709\u7528\u5f62\u8cea\u3092\u6301\u3063\u305f\u6539\u5909\u682a\u306e\u4f5c\u51fa\u304c\u6c42\u3081\u3089\u308c\u3066\u3044\u307e\u3059\u3002
\n\u3000<\/strong>\u3057\u304b\u3057\u3001\u5b9f\u7528\u7684\u304b\u3064\u6301\u7d9a\u7684\u306a\u30df\u30c9\u30ea\u30e0\u30b7\u306e\u907a\u4f1d\u5b50\u6a5f\u80fd\u6539\u5909\u6cd5\u306f\u3001\u307e\u3060\u78ba\u7acb\u3055\u308c\u3066\u304a\u3089\u305a\u3001\u305d\u308c\u304c\u57fa\u790e\u7814\u7a76\u3084\u52b9\u7387\u7684\u306a\u80b2\u7a2e\u3092\u884c\u3046\u4e0a\u3067\u306e\u59a8\u3052\u3068\u306a\u3063\u3066\u3044\u307e\u3057\u305f\u3002\u305d\u3053\u3067\u3001\u7814\u7a76\u30c1\u30fc\u30e0\u306f\u3001\u5c06\u6765\u7684\u306a\u7523\u696d\u5229\u7528\u306e\u53ef\u80fd\u6027\u3082\u8e0f\u307e\u3048\u3066\u3001\u5b9f\u7528\u9014\u306b\u5373\u3057\u305f\u30b2\u30ce\u30e0\u7de8\u96c6\u65b9\u6cd5\u306e\u78ba\u7acb\u3092\u76ee\u6307\u3057\u3066\u7814\u7a76\u3092\u958b\u59cb\u3057\u307e\u3057\u305f\u3002<\/p>\n

\"\u56f3\uff12\"<\/p>\n

\u56f31\u3000\u30df\u30c9\u30ea\u30e0\u30b7\uff08Euglena gracilis\uff09\u306e\u597d\u6c17\u6761\u4ef6\u3067\u306e\u57f9\u990a\u306e\u69d8\u5b50\u3068\u9855\u5fae\u93e1\u5199\u771f<\/p>\n

(A) \u5b9f\u9a13\u5ba4\u3067\u6db2\u4f53\u57f9\u5730\u3092\u7528\u3044\u3066\u632f\u3068\u3046\u57f9\u990a\u3057\u305f\u30df\u30c9\u30ea\u30e0\u30b7\u3002
\n(B) \u57f9\u990a\u6db2\u4e2d\u3067\u5897\u6b96\u3057\u3066\u3044\u308b\u30df\u30c9\u30ea\u30e0\u30b7\u306e\u69d8\u5b50\u3002\u30b9\u30b1\u30fc\u30eb\u30d0\u30fc\u306f100 \u30de\u30a4\u30af\u30ed\u30e1\u30fc\u30c8\u30eb\uff08\u03bcm\u30011,000\u5206\u306e1mm\uff09\u3002
\n(C) \u30df\u30c9\u30ea\u30e0\u30b7\uff11\u500b\u4f53\u306e\u9855\u5fae\u93e1\u50cf\u3002\u7d30\u80de\u5185\u306b\u591a\u6570\u307f\u3089\u308c\u308b\u5c0f\u3055\u306a\u7c92\u72b6\u306e\u3082\u306e\u304c\u30d1\u30e9\u30df\u30ed\u30f3\u7c92\u3067\u3042\u308b\u3002\u30b9\u30b1\u30fc\u30eb\u30d0\u30fc\u306f10 \u03bcm\u3002<\/span><\/p>\n

\u00a0\uff12\uff0e\u7814\u7a76\u624b\u6cd5\u3068\u6210\u679c<\/strong><\/p>\n

\u3000<\/strong>\u7814\u7a76\u30c1\u30fc\u30e0\u306f\u307e\u305a\u3001\u30df\u30c9\u30ea\u30e0\u30b7\u306e\u30d1\u30e9\u30df\u30ed\u30f3\u5408\u6210\u306b\u95a2\u308f\u308b\u9175\u7d20\u306e\u907a\u4f1d\u5b50EgGSL2 <\/em>[6]<\/sup>\u3092\u6a19\u7684\u3068\u3057\u305f\u30ac\u30a4\u30c9RNA\uff08gRNA\uff09[3]<\/sup>\u3092\u8a2d\u8a08\u3057\u3001\u6a19\u7684DNA\u306e\u5207\u65ad\u6d3b\u6027\u3092\u6301\u3063\u305fCas9 RNP\u8907\u5408\u4f53\u3092\u4f5c\u88fd\u3057\u307e\u3057\u305f\u3002\u6b21\u306b\u3001\u30d1\u30e9\u30df\u30ed\u30f3\u7c92\u306e\u5f62\u614b\u5909\u5316\u3084T7E1 assay\u6cd5[7]<\/sup>\u3092\u6307\u6a19\u3068\u3057\u3001\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u6cd5[8]<\/sup>\u3092\u7528\u3044\u3066\u3001\u30df\u30c9\u30ea\u30e0\u30b7\u7d30\u80de\u5185\u306bCas9 RNP\u8907\u5408\u4f53\u3092\u76f4\u63a5\u5c0e\u5165\u3059\u308b\u305f\u3081\u306e\u6700\u9069\u306a\u8af8\u6761\u4ef6\u3092\u63a2\u308a\u307e\u3057\u305f\uff08\u56f32, 3\uff09\u3002
\n\u3000<\/strong>\u305d\u306e\u7d50\u679c\u3001\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u89e3\u6790[9]<\/sup>\u306b\u3088\u308b\u8a55\u4fa1\u306b\u304a\u3044\u3066\u3001Cas9 RNP\u8907\u5408\u4f53\u3092\u5c0e\u5165\u5f8c72\u6642\u9593\u3067\u7d0480 \uff05\u3068\u3044\u3046\u3001\u4ed6\u306e\u5fae\u7d30\u85fb\u985e\u3067\u306f\u985e\u3092\u898b\u306a\u3044\u307b\u3069\u306e\u9ad8\u52b9\u7387\u3067\u306e\u30e9\u30f3\u30c0\u30e0\u306a\u6b20\u640d\u30fb\u633f\u5165\u5909\u7570\u5c0e\u5165\u306b\u6210\u529f\u3057\u307e\u3057\u305f\u3002<\/p>\n

\"\u56f3\uff13\"<\/p>\n

\u56f32\u3000Cas9 RNP\u8907\u5408\u4f53\u3092\u7528\u3044\u305f\u30df\u30c9\u30ea\u30e0\u30b7\uff08Euglena gracilis\uff09\u30b2\u30ce\u30e0\u7de8\u96c6\u624b\u6cd5\u306e\u6d41\u308c<\/p>\n

\u307e\u305a\u3001\u30df\u30c9\u30ea\u30e0\u30b7\u306e\u6a19\u7684\u907a\u4f1d\u5b50EgGSL2\u306b\u5bfe\u5fdc\u3059\u308bgRNA\uff08crRNA\u3068tracrRNA\uff09\u3092\u8a2d\u8a08\u3057\u3001Cas9 RNP\u8907\u5408\u4f53\u3092\u4f5c\u88fd\u3059\u308b\u3002\u3053\u308c\u3092\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u6cd5\u306b\u3066\u30df\u30c9\u30ea\u30e0\u30b7\u306b\u76f4\u63a5\u5c0e\u5165\u3059\u308b\u3002\u3053\u306e\u3068\u304d\u3001\u6700\u9069\u5316\u3055\u305b\u305f\u6761\u4ef6\u3092\u7528\u3044\u308b\u3053\u3068\u3067\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u500b\u4f53\u3092\u9ad8\u52b9\u7387\u304b\u3064\u77ed\u6642\u9593\u3067\u53d6\u5f97\u53ef\u80fd\u3068\u306a\u3063\u305f\u3002<\/span><\/p>\n

\"\u56f34\"<\/p>\n

\u56f33\u3000Cas9 RNP\u8907\u5408\u4f53\u3092\u7528\u3044\u305f\u30b2\u30ce\u30e0\u7de8\u96c6\u306b\u3088\u308a\u4f5c\u51fa\u3057\u305f\u30df\u30c9\u30ea\u30e0\u30b7\uff08Euglena gracilis\uff09\u306e\u4f8b<\/p>\n

(A)\u30df\u30c9\u30ea\u30e0\u30b7\u306e\u91ce\u751f\u682a\u3068\u3001(B) \u30d1\u30e9\u30df\u30ed\u30f3\u5408\u6210\u306b\u95a2\u308f\u308b\u9175\u7d20\u306eEgGSL2<\/em>\u907a\u4f1d\u5b50\u3092\u6a19\u7684\u3068\u3059\u308bCas9 RNP\u8907\u5408\u4f53\u3092\u5c0e\u5165\u3057\u3001\u4f5c\u51fa\u3057\u305f\u30b2\u30ce\u30e0\u7de8\u96c6\u682a\u306e\u4e00\u4f8b\u3002B\u306e\u30df\u30c9\u30ea\u30e0\u30b7\u3067\u306f\u3001EgGSL2<\/em>\u907a\u4f1d\u5b50\u306e\u6a5f\u80fd\u304c\u5931\u308f\u308c\u3066\u3044\u308b\u305f\u3081\u3001\u91ce\u751f\u682a\u306b\u304a\u3044\u3066\u5f93\u5c5e\u6804\u990a\u57f9\u990a\u4e0b\u3067\u751f\u3058\u308b\u30d1\u30e9\u30df\u30ed\u30f3\u7c92\u306e\u84c4\u7a4d\u304c\u307b\u3068\u3093\u3069\u898b\u3089\u308c\u306a\u3044\u3002 \u30b9\u30b1\u30fc\u30eb\u30d0\u30fc\u306f10 \u03bcm\u3002<\/span><\/p>\n

\u3000<\/strong>\u307e\u305f\u3001\u30b2\u30ce\u30e0\u4e0a\u306e\u96e2\u308c\u305f\u4f4d\u7f6e\u3092\u6a19\u7684\u3068\u3059\u308b2\u7a2e\u985e\u306eCas9 RNP\u3092\u30df\u30c9\u30ea\u30e0\u30b7\u306e\u7d30\u80de\u5185\u306b\u540c\u6642\u306b\u5c0e\u5165\u3059\u308b\u3053\u3068\u3067\u3001\u9577\u3044\u6b20\u640d\u5909\u7570\u3092\u8a98\u767a\u3067\u304d\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u3001Cas9 RNP\u8907\u5408\u4f53\u3068\u4e00\u7dd2\u306b\u3001\u6a19\u7684\u914d\u5217\u90e8\u4f4d\u4e0a\u4e0b\u6d41\u3068\u76f8\u540c\u306a\u914d\u5217\u3092\u4e21\u7aef\u90e8\u306b\u4ed8\u52a0\u3057\u305fssODN \uff08\u4e00\u672c\u9396\u30aa\u30ea\u30b4\u30c7\u30aa\u30ad\u30b7\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\uff09[10]\u3092\u3001\u30c9\u30ca\u30fcDNA\u3068\u3057\u3066\u30df\u30c9\u30ea\u30e0\u30b7\u306e\u7d30\u80de\u5185\u306b\u5c0e\u5165\u3059\u308b\u3053\u3068\u3067\u3001\u6b63\u78ba\u306a\u5c0f\u898f\u6a21\uff08\u77ed\u3044\u5869\u57fa\u914d\u5217\uff09\u306e\u30ce\u30c3\u30af\u30a4\u30f3[11]\u306b\u3082\u6210\u529f\u3057\u307e\u3057\u305f\u3002<\/p>\n

\uff13\uff0e\u4eca\u5f8c\u306e\u671f\u5f85<\/strong><\/p>\n

\u3000<\/strong>\u672c\u7814\u7a76\u3067\u306f\u3001\u4ed6\u306e\u5fae\u7d30\u85fb\u985e\u3067\u306f\u985e\u3092\u898b\u306a\u3044\u307b\u3069\u306e\u9ad8\u3044\u52b9\u7387\u3067\u3001\u30df\u30c9\u30ea\u30e0\u30b7\u306b\u304a\u3051\u308bDNA\u30d5\u30ea\u30fc\u30b2\u30ce\u30e0\u7de8\u96c6\u624b\u6cd5\u306e\u78ba\u7acb\u306b\u6210\u529f\u3057\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u3001\u4e0a\u8a18\u306e\u9ad8\u52b9\u7387\u306a\u30b2\u30ce\u30e0\u7de8\u96c6\u6cd5\u3092\u30d9\u30fc\u30b9\u306b\u3001ssODN\u3092\u7528\u3044\u305f\u52b9\u7387\u7684\u306a\u30ce\u30c3\u30af\u30a4\u30f3\u5b9f\u9a13\u306b\u3082\u6210\u529f\u3057\u3001\u3088\u308a\u6b63\u78ba\u306b\u5236\u5fa1\u3055\u308c\u305f\u30df\u30c9\u30ea\u30e0\u30b7\u30b2\u30ce\u30e0\u306e\u6539\u5909\u3082\u53ef\u80fd\u3068\u306a\u308a\u307e\u3057\u305f\u3002
\n\u3000<\/strong>\u4eca\u5f8c\u3001\u672c\u6280\u8853\u3092\u99c6\u4f7f\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u30df\u30c9\u30ea\u30e0\u30b7\u306b\u304a\u3051\u308b\u907a\u4f1d\u5b50\u6a5f\u80fd\u306e\u89e3\u6790\u306a\u3069\u306e\u57fa\u790e\u7814\u7a76\u306e\u63a8\u9032\u306b\u52a0\u3048\u3001\u6709\u7528\u7269\u8cea\u751f\u7523\u80fd\u3092\u5411\u4e0a\u3055\u305b\u305f\u682a\u306e\u5206\u5b50\u80b2\u7a2e\u3084\u76ee\u7684\u306b\u5fdc\u3058\u3066\u30c7\u30b6\u30a4\u30f3\u3057\u305f\u30df\u30c9\u30ea\u30e0\u30b7\u306e\u4f5c\u51fa\u304c\u53ef\u80fd\u3068\u306a\u308b\u3068\u671f\u5f85\u3067\u304d\u307e\u3059\u3002
\n\u3000<\/strong>\u307e\u305f\u672c\u7814\u7a76\u306f\u3001\u56fd\u969b\u9023\u5408\u304c2016\u5e74\u306b\u5b9a\u3081\u305f17\u9805\u76ee\u306e\u300c\u6301\u7d9a\u53ef\u80fd\u306a\u958b\u767a\u76ee\u6a19 (SDGs)\u300d\u306e\u3046\u3061\u300c7.\u30a8\u30cd\u30eb\u30ae\u30fc\u3092\u307f\u3093\u306a\u306b\u305d\u3057\u3066\u30af\u30ea\u30fc\u30f3\u306b\u300d\u3068\u300c13.\u6c17\u5019\u5909\u52d5\u306b\u5177\u4f53\u7684\u306a\u5bfe\u7b56\u3092\u300d\u3078\u306e\u8ca2\u732e\u304c\u671f\u5f85\u3055\u308c\u308b\u6210\u679c\u3067\u3059\u3002<\/p>\n

\uff14\uff0e\u8ad6\u6587\u60c5\u5831<\/strong><\/p>\n

\uff1c\u30bf\u30a4\u30c8\u30eb\uff1e
\nHighly efficient transgene-free targeted mutagenesis and single-stranded oligodeoxynucleotide-mediated precise knock-in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins
\n\uff1c\u8457\u8005\u540d\uff1e
\nToshihisa Nomura, Komaki Inoue, Yukiko Uehara-Yamaguchi, Koji Yamada, Osamu Iwata, Kengo Suzuki, Keiichi Mochida
\n\uff1c\u96d1\u8a8c\uff1e
\nPlant Biotechnology Journal
\n<\/em>\uff1cDOI\uff1e
\n10.1111\/pbi.13174<\/p>\n

\uff15\uff0e\u88dc\u8db3\u8aac\u660e<\/strong><\/p>\n

[1] Euglena gracilis
\n\u7530\u3093\u307c\u3084\u6de1\u6c34\u306e\u6e56\u6cbc\u306a\u3069\u306b\u751f\u80b2\u3059\u308b\u30e6\u30fc\u30b0\u30ec\u30ca\u5c5e\u306e\u5fae\u7d30\u85fb\u985e\u306e\u4e00\u7a2e\u3067\u3001\u53e4\u304f\u304b\u3089\u751f\u7269\u5b66\u5b9f\u9a13\u306b\u4f7f\u7528\u3055\u308c\u3066\u3044\u308b\u3002\u672c\u7a2e\u306f\u3001\u5927\u91cf\u57f9\u990a\u6cd5\u304c\u78ba\u7acb\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u304b\u3089\u3001\u30df\u30c9\u30ea\u30e0\u30b7\u306e\u4e2d\u3067\u6700\u3082\u7523\u696d\u5229\u7528\u306b\u9069\u3057\u3066\u304a\u308a\u3001\u3055\u307e\u3056\u307e\u306a\u7528\u9014\u3067\u306e\u5229\u6d3b\u7528\u304c\u5c55\u958b\u3055\u308c\u3066\u3044\u308b\u3002<\/p>\n

[2] \u30b2\u30ce\u30e0\u7de8\u96c6
\n\u6838\u9178\u5206\u89e3\u9175\u7d20\uff08\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\uff09\u306a\u3069\u3092\u90e8\u4f4d\u7279\u7570\u7684\u306b\u4f5c\u7528\u3055\u305b\u308b\u3053\u3068\u3067\u3001\u907a\u4f1d\u60c5\u5831\u3092\u6539\u5909\u3059\u308b\u6280\u8853\u3002<\/p>\n

[3] Cas9 RNP\u8907\u5408\u4f53\u3001\u30ac\u30a4\u30c9RNA\uff08gRNA\uff09
\nCas9 RNP\u8907\u5408\u4f53\u306f\u3001\u30b2\u30ce\u30e0\u4e0a\u306e\u6a19\u7684\u7b87\u6240\u306b\u57fa\u3065\u3044\u3066\u8a2d\u8a08\u3057\u305fgRNA\u3068Cas9\u30bf\u30f3\u30d1\u30af\u8cea\u304b\u3089\u69cb\u6210\u3055\u308c\u308b\u5b89\u5b9a\u7684\u306a\u30ea\u30dc\u6838\u30bf\u30f3\u30d1\u30af\u8cea\u8907\u5408\u4f53\u3067\u3001gRNA\u306b\u5bfe\u5fdc\u3059\u308b\u30b2\u30ce\u30e0\u4e0a\u306e\u6a19\u7684DNA\u30b5\u30a4\u30c8\u3092\u7279\u7570\u7684\u306b\u5207\u65ad\u3059\u308b\u3002\u30b2\u30ce\u30e0\u7de8\u96c6\u306b\u304a\u3051\u308bgRNA \u306f\u3001crRNA\uff08CRISPR RNA\uff09\u3068tracrRNA\uff08trans-activating crRNA\uff09\u3042\u308b\u3044\u306fsingle guide RNA \u3092\u6307\u3059\u3002Cas9 RNP\u306f\u3001Cas9 (CRISPR associated protein 9)\/gRNA (guide RNA) Ribonucleoproteins \u306e\u7565\u3002<\/p>\n

[4] \u6301\u7d9a\u53ef\u80fd\u306a\u958b\u767a\u76ee\u6a19\uff08SDGs\uff09
\n2015\u5e749\u6708\u306e\u56fd\u9023\u30b5\u30df\u30c3\u30c8\u3067\u63a1\u629e\u3055\u308c\u305f\u300c\u6301\u7d9a\u53ef\u80fd\u306a\u958b\u767a\u306e\u305f\u3081\u306e2030\u30a2\u30b8\u30a7\u30f3\u30c0\u300d\u306b\u3066\u8a18\u8f09\u3055\u308c\u305f2016\u5e74\u304b\u30892030\u5e74\u307e\u3067\u306e\u56fd\u969b\u76ee\u6a19\u3002\u6301\u7d9a\u53ef\u80fd\u306a\u4e16\u754c\u3092\u5b9f\u73fe\u3059\u308b\u305f\u3081\u306e17\u306e\u30b4\u30fc\u30eb\u3001169\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u304b\u3089\u69cb\u6210\u3055\u308c\u3001\u767a\u5c55\u9014\u4e0a\u56fd\u306e\u307f\u306a\u3089\u305a\uff0c\u5148\u9032\u56fd\u81ea\u8eab\u304c\u53d6\u308a\u7d44\u3080\u30e6\u30cb\u30d0\u30fc\u30b5\u30eb\uff08\u666e\u904d\u7684\uff09\u306a\u3082\u306e\u3067\u3042\u308a\u3001\u65e5\u672c\u3068\u3057\u3066\u3082\u7a4d\u6975\u7684\u306b\u53d6\u308a\u7d44\u3093\u3067\u3044\u308b\uff08\u5916\u52d9\u7701\u30db\u30fc\u30e0\u30da\u30fc\u30b8\u304b\u3089\u4e00\u90e8\u6539\u5909\u3057\u3066\u8ee2\u8f09\uff09<\/p>\n

[5] \u30d1\u30e9\u30df\u30ed\u30f3
\n\u30b0\u30eb\u30b3\u30fc\u30b9\uff08\u30d6\u30c9\u30a6\u7cd6\uff09\u304c\u03b2-1,3\u7d50\u5408\u3057\u305f\u3001\u30df\u30c9\u30ea\u30e0\u30b7\u306b\u304a\u3051\u308b\u8caf\u8535\u591a\u7cd6\u985e\u3002\u30df\u30c9\u30ea\u30e0\u30b7\u306e\u7d30\u80de\u5185\u3067\u84c4\u7a4d\u3059\u308b\u3068\u3001\u7c92\u72b6\u306e\u69cb\u9020\u4f53\u3068\u3057\u3066\u898b\u3089\u308c\u308b\u3002\u307e\u305f\u3001\u5acc\u6c17\u6761\u4ef6\u4e0b\u3067\u306f\u3001\u30a8\u30cd\u30eb\u30ae\u30fc\u7372\u5f97\u306e\u305f\u3081\u3001\u30d1\u30e9\u30df\u30ed\u30f3\u3092\u3082\u3068\u306b\u6cb9\u8102\uff08\u30ef\u30c3\u30af\u30b9\u30a8\u30b9\u30c6\u30eb\uff09\u304c\u751f\u7523\u3055\u308c\u308b\u3002<\/p>\n

[6] EgGSL2\u907a\u4f1d\u5b50
\n\u30df\u30c9\u30ea\u30e0\u30b7\u306e\u30d1\u30e9\u30df\u30ed\u30f3\u5408\u6210\u306b\u95a2\u4e0e\u3059\u308b\u9175\u7d20\u907a\u4f1d\u5b50\u3002\u3053\u306e\u907a\u4f1d\u5b50\u3092\u6a5f\u80fd\u963b\u5bb3\u3059\u308b\u3068\u3001\u5f93\u5c5e\u6804\u990a\u57f9\u990a\u6761\u4ef6\u3067\u306e\u30d1\u30e9\u30df\u30ed\u30f3\u84c4\u7a4d\u304c\u4f4e\u4e0b\u3059\u308b\u3002EgGSL2\u306fE. gracilis Glucan Synthase-Like 2\u306e\u7565\u3002<\/p>\n

[7] T7EI assay
\nDNA\u306e\u30df\u30b9\u30de\u30c3\u30c1\u3092\u8a8d\u8b58\u3057\u3066\u5207\u65ad\u3059\u308b\u9175\u7d20\u3067\u3042\u308bT7 Endonuclease I \uff08T7EI\uff09\u3092\u7528\u3044\u3066\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u306a\u3069\u306b\u3088\u308b\u6b20\u5931\u30fb\u633f\u5165\u5909\u7570\u306e\u6709\u7121\u3092\u7c21\u6613\u7684\u306b\u691c\u51fa\u3059\u308b\u624b\u6cd5\u3002<\/p>\n

[8] \u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u6cd5
\n\u96fb\u6c17\u7a7f\u5b54\u6cd5\u3068\u3082\u547c\u3070\u308c\u3001\u96fb\u6c17\u30d1\u30eb\u30b9\u306b\u3088\u308a\u7d30\u80de\u819c\u306b\u5fae\u5c0f\u306a\u7a74\u3092\u4e00\u6642\u7684\u306b\u958b\u3051\u3001\u7269\u8cea\uff08\u672c\u7814\u7a76\u306e\u5834\u5408\u3001Cas9 RNP\u8907\u5408\u4f53\u304a\u3088\u3073ssODN\uff09\u3092\u76f4\u63a5\u7684\u306b\u5c0e\u5165\u3059\u308b\u624b\u6cd5\u3002<\/p>\n

[9] \u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u89e3\u6790
\n\u30b2\u30ce\u30e0\u4e0a\u306e\u7279\u5b9a\u306e\u7b87\u6240\uff08\u672c\u7814\u7a76\u3067\u306fEgGSL2\u907a\u4f1d\u5b50\u5185\u306e\u6a19\u7684\u30b5\u30a4\u30c8\uff09\u3092\u5897\u5e45\u3057\u3001\u305d\u308c\u3089\u306e\u5869\u57fa\u914d\u5217\u60c5\u5831\u3092\u3001\u6b21\u4e16\u4ee3\u30b7\u30fc\u30af\u30a8\u30f3\u30b5\u30fc\u3092\u7528\u3044\u3066\u7db2\u7f85\u7684\u306b\u53d6\u5f97\u3059\u308b\u89e3\u6790\u624b\u6cd5\u3002<\/p>\n

[10] ssODN \uff08\u4e00\u672c\u9396\u30aa\u30ea\u30b4\u30c7\u30aa\u30ad\u30b7\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\uff09
\n\u4e00\u672c\u9396\u306eDNA\u3002ssODN\u3092\u30c9\u30ca\u30fcDNA\u3068\u3057\u3066\u3001RNP\u8907\u5408\u4f53\u306a\u3069\u3068\u4e00\u7dd2\u306b\u7d30\u80de\u5185\u306b\u5c0e\u5165\u3059\u308b\u3053\u3068\u3067\u3001\u76f8\u540c\u6027\u4fee\u5fa9\u6a5f\u69cb\u306b\u57fa\u3065\u304f\u30b2\u30ce\u30e0\u3078\u306e\u30ce\u30c3\u30af\u30a4\u30f3\u3084\u5869\u57fa\u7f6e\u63db\u304c\u53ef\u80fd\u3068\u306a\u308b\u3002<\/p>\n

[11] \u30ce\u30c3\u30af\u30a4\u30f3
\n\u30b2\u30ce\u30e0\u4e0a\u306e\u6a19\u7684\u90e8\u4f4d\u306bDNA\u3092\u633f\u5165\u3059\u308b\u907a\u4f1d\u5b50\u5de5\u5b66\u7684\u306a\u624b\u6cd5\u3002<\/p>\n

\uff16\uff0e\u767a\u8868\u8005\u30fb\u6a5f\u95a2\u7a93\u53e3<\/strong><\/p>\n

\uff1c\u767a\u8868\u8005\uff1e\u3000\u203b\u7814\u7a76\u5185\u5bb9\u306b\u3064\u3044\u3066\u306f\u767a\u8868\u8005\u306b\u304a\u554f\u3044\u5408\u308f\u305b\u4e0b\u3055\u3044
\n\u7406\u5316\u5b66\u7814\u7a76\u6240
\n\u3000<\/strong>\u74b0\u5883\u8cc7\u6e90\u79d1\u5b66\u7814\u7a76\u30bb\u30f3\u30bf\u30fc
\n\u3000\u3000<\/strong>\u30d0\u30a4\u30aa\u751f\u7523\u60c5\u5831\u7814\u7a76\u30c1\u30fc\u30e0
\n\u3000\u3000\u3000<\/strong>\u7814\u7a76\u54e1\u3000\u91ce\u6751\u3000\u4fca\u5c1a\u3000\uff08\u306e\u3080\u3089\u3000\u3068\u3057\u3072\u3055\uff09
\n\u3000\u3000\u3000<\/strong>\u30c1\u30fc\u30e0\u30ea\u30fc\u30c0\u30fc\u3000\u6301\u7530\u3000\u6075\u4e00\u3000\uff08\u3082\u3061\u3060\u3000\u3051\u3044\u3044\u3061\uff09<\/p>\n

\u79d1\u6280\u30cf\u30d6\u7523\u9023\u672c\u90e8\u3000\u30d0\u30c8\u30f3\u30be\u30fc\u30f3\u7814\u7a76\u63a8\u9032\u30d7\u30ed\u30b0\u30e9\u30e0
\n\u3000<\/strong>\u5fae\u7d30\u85fb\u985e\u751f\u7523\u5236\u5fa1\u6280\u8853\u7814\u7a76\u30c1\u30fc\u30e0
\n\u3000\u3000<\/strong>\u30c1\u30fc\u30e0\u30ea\u30fc\u30c0\u30fc\u3000\u9234\u6728\u3000\u5065\u543e\u3000\uff08\u3059\u305a\u304d\u3000\u3051\u3093\u3054\uff09
\n\u3000\u3000<\/strong>\uff08\u682a\u5f0f\u4f1a\u793e\u30e6\u30fc\u30b0\u30ec\u30ca\u3000\u57f7\u884c\u5f79\u54e1\u7814\u7a76\u958b\u767a\u62c5\u5f53\/\u5148\u7aef\u6280\u8853\u7814\u7a76\u90e8\u9577\uff09<\/p>\n

TEL\uff1a045-503-7077\uff08\u91ce\u6751\u3001\u6301\u7530\uff09\u3000FAX\uff1a045-503-9609\uff08\u91ce\u6751\u3001\u6301\u7530\uff09
\nE-mail\uff1atoshihisa.nomura[at]riken.jp\uff08\u91ce\u6751\uff09, keiichi.mochida[at]riken.jp\uff08\u6301\u7530\uff09<\/p>\n

\"6\u56f3\"<\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

\u2015\u5831\u9053\u95a2\u4fc2\u8005\u304a\u554f\u3044\u5408\u308f\u305b\u5148\u2015<\/strong>
\n\u682a\u5f0f\u4f1a\u793e\u30e6\u30fc\u30b0\u30ec\u30ca\u3000\u30b3\u30fc\u30dd\u30ec\u30fc\u30c8\u30b3\u30df\u30e5\u30cb\u30b1\u30fc\u30b7\u30e7\u30f3\u8ab2
\n\u7406\u5316\u5b66\u7814\u7a76\u6240\u3000\u5e83\u5831\u5ba4\u3000\u5831\u9053\u62c5\u5f53<\/p>\n","protected":false},"excerpt":{"rendered":"

\u30df\u30c9\u30ea\u30e0\u30b7\u3067\u306e\u9ad8\u52b9\u7387\u30b2\u30ce\u30e0\u7de8\u96c6\u306b\u6210\u529f \uff5e\u30d0\u30a4\u30aa\u71c3\u6599\u751f\u7523\u306a\u3069\u306b\u5411\u3051\u305f\u6709\u7528\u30df\u30c9\u30ea\u30e0\u30b7\u80b2\u7a2e\u3078\u671f\u5f85\uff5e \u682a\u5f0f\u4f1a\u793e\u30e6\u30fc\u30b0\u30ec\u30ca \u7406\u5316\u5b66\u7814\u7a76\u6240 \u3000\u7406\u5316\u5b66\u7814\u7a76\u6240\uff08\u7406\u7814\uff09\u74b0\u5883\u8cc7\u6e90\u79d1\u5b66\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u30d0\u30a4\u30aa\u751f\u7523\u60c5\u5831\u7814\u7a76\u30c1\u30fc\u30e0 \u306e\u91ce\u6751\u4fca\u5c1a\u7814\u7a76\u54e1\u3001\u6301 […]<\/p>\n","protected":false},"author":10,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[2],"tags":[9,10],"acf":[],"_links":{"self":[{"href":"https:\/\/www.euglena.jp\/news\/wp-json\/wp\/v2\/posts\/16585"}],"collection":[{"href":"https:\/\/www.euglena.jp\/news\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.euglena.jp\/news\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.euglena.jp\/news\/wp-json\/wp\/v2\/users\/10"}],"replies":[{"embeddable":true,"href":"https:\/\/www.euglena.jp\/news\/wp-json\/wp\/v2\/comments?post=16585"}],"version-history":[{"count":20,"href":"https:\/\/www.euglena.jp\/news\/wp-json\/wp\/v2\/posts\/16585\/revisions"}],"predecessor-version":[{"id":17082,"href":"https:\/\/www.euglena.jp\/news\/wp-json\/wp\/v2\/posts\/16585\/revisions\/17082"}],"wp:attachment":[{"href":"https:\/\/www.euglena.jp\/news\/wp-json\/wp\/v2\/media?parent=16585"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.euglena.jp\/news\/wp-json\/wp\/v2\/categories?post=16585"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.euglena.jp\/news\/wp-json\/wp\/v2\/tags?post=16585"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}